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AKT1, p Ser473 Antibody (17F6.B11), DyLight 488, Novus Biologicals™
SDP

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NBP177701 0.1 mg
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Catalog No. NBP177701 Supplier Novus Biologicals Supplier No. NBP177701
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Description

Mouse Monoclonal Antibody

AKT1 Monoclonal specifically detects AKT1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Fluorophore-linked immunosorbent assay.

Specifications

Antigen AKT1 (p Ser473)
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone 17F6.B11
Concentration LYOPH
Conjugate DyLight 488
Dilution Western Blot 1:500 - 1:3000, Flow Cytometry 1:10 - 1:1000, ELISA 1:20000, Immunohistochemistry 20 ug/mL, Immunocytochemistry/Immunofluorescence >1:5000, Immunohistochemistry-Paraffin 20 ug/mL, Fluorophore-linked immunosorbent assay 1:100-1:2000
Gene Accession No. P31749
Gene Alias AKT, AKT serine/threonine kinase 1, AKT1m, CWS6, EC 2.7.11, EC 2.7.11.1, PKB, PKB alpha, PKBMGC99656, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, rac protein kinase alpha, RAC-ALPHA, RAC-alpha serine/threonine-protein kinas
Gene Symbols AKT1
Host Species Mouse
Immunogen AKT1 [p Ser473] Antibody (17F6.B11) was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT11 protein, followed by hybridoma development. (Uniprot: P31749)
Molecular Weight of Antigen 55.7 kDa
Purification Method Protein A purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Apoptosis, Autophagy, Cancer, Growth and Development, Hypoxia, mTOR Pathway, Neuronal Cell Markers, Phospho Specific, Signal Transduction
Primary or Secondary Primary
Gene ID (Entrez) 207
Test Specificity This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Reconstitution Reconstitute with 100 ul deionized water (or equivalent)
Target Species Human, Mouse, Rat, Monkey
Content And Storage Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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