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AKT1, p Ser473 Antibody (17F6.B11), Novus Biologicals™
SDP

Catalog No. NBP169923 Shop All R&D Systems Products
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NBP169923 0.1 mg
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Catalog No. NBP169923 Supplier Novus Biologicals Supplier No. NBP169923
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Description

Mouse Monoclonal Antibody has been used in 2 publications

AKT1 Monoclonal specifically detects AKT1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

Antigen AKT1 (p Ser473)
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Dot Blot
Classification Monoclonal
Clone 17F6.B11
Concentration 1 mg/ml
Conjugate Unconjugated
Dilution Western Blot 1:500-1:3000, Flow Cytometry 1:10-1:1000, ELISA 1:20000, Immunohistochemistry 20 ug/ml, Immunocytochemistry/Immunofluorescence 1:500-1:3000, Immunoprecipitation, Immunohistochemistry-Paraffin 1:10-1:500, Dot Blot
Gene Accession No. P31749
Gene Alias AKT, AKT serine/threonine kinase 1, AKT1m, CWS6, EC 2.7.11, EC 2.7.11.1, PKB, PKB alpha, PKBMGC99656, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, rac protein kinase alpha, RAC-ALPHA, RAC-alpha serine/threonine-protein kinas
Gene Symbols AKT1
Host Species Mouse
Immunogen AKT1 [p Ser473] Antibody (17F6.B11) was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT11 protein, followed by hybridoma development. (Uniprot: P31749)
Molecular Weight of Antigen 55.7 kDa
Purification Method Protein A purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Apoptosis, Autophagy, Cancer, Growth and Development, Hypoxia, mTOR Pathway, Neuronal Cell Markers, Signal Transduction
Primary or Secondary Primary
Gene ID (Entrez) 207
Test Specificity This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Target Species Human, Mouse, Rat, Monkey
Content And Storage Store at -20C. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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For Research Use Only

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