ALK, Mouse anti-Human, Clone: ALK1, Millipore Sigma™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABC117125	MilliporeSigma MABC117125UG	25 μg
MABC1171100	MilliporeSigma MABC1171100UG	100 μg

Description

Anti-ALK, clone ALK1, Cat. No. MABC1171, is a mouse monoclonal antibody that detects ALK tyrosine kinase receptor and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

ALK tyrosine kinase receptor (UniProt: Q9UM73; also known as EC: 2.7.10.1, Anaplastic lymphoma kinase, CD246) is encoded by the ALK gene (Gene ID: 238) in human. ALK tyrosine kinase receptor is a single-pass type I membrane protein that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the development and function of the nervous system. It is predominantly expressed in the central nervous system, intestine, and testis. It acts as a receptor for ligands pleiotrophin (PTN) and midkine (MDK). It is activated and homodimerizes by ligand-binding and subsequent phosphorylation. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. It is inactivated through dephosphorylation by receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) in the absence of any stimulation by a ligand. A chromosomal aberration involving ALK has been reported in non-Hodgkin lymphoma where the kinase is constitutively activated. Another chromosomal aberration involving ALK has also been linked with anaplastic large-cell lymphoma (ALCL). ALK tyrosine kinase receptor is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off in the mature form. ALK tyrosine kinase receptor consists of an extracellular domain (aa 19-1038); a transmembrane domain (aa 1039-1059); and a cytoplasmic domain (aa 1060-1620).

Specifications

• Antigen: ALK
• Dilution: Western Blotting Analysis: A representative lot detected ALK in Western Blotting applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunohistochemistry Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunofluorescence Analysis: A representative lot detected ALK in Immunofluorescence applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunoprecipitation Analysis: A representative lot immunoprecipitated ALK in Immunoprecipitation applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunohistochemistry (Paraffin) Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: ALK tyrosine kinase receptor;EC: 2.7.10.1;Anaplastic lymphoma kinase;CD246
• Gene Symbols: ALK
• Isotype: IgG3 κ
• Purification Method: Protein G purified
• Test Specificity: Clone ALK1 specifically detects human ALK tyrosine kinase receptor. It targets an epitope with in 102 amino acids from the C-terminal region.

• Clone: ALK1
• Applications: Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Signaling
• Formulation: Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene ID (Entrez): NP_004295
• Immunogen: His-tagged full length human recombinant ALK tyrosine kinase receptor.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.