Alkaline Phosphatase/ALPP Antibody (ALPP/238), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2449560.2MG

Catalog No. NBP24495602

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence

Alkaline Phosphatase/ALPP Monoclonal antibody specifically detects Alkaline Phosphatase/ALPP in Human samples. It is validated for Flow Cytometry, Immunohistochemistry-Frozen, SDS-Page, Immunofluorescence.

Specifications

• Antigen: Alkaline Phosphatase/ALPP
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 1.0 - 2.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P05187
• Gene Symbols: ALPP
• Immunogen: Recombinant full-length human ALPP protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This MAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP as to AP from normal human testis. This MAb can be used both as coating as well as tracer antibody in the same ELISA to detect PLAP in serum of S, F, FS and I phenotypes.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), SDS-Page, Immunofluorescence
• Clone: ALPP/238
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: Alkaline phosphatase Regan isozyme, alkaline phosphatase, placental, alkaline phosphatase, placental (Regan isozyme), alkaline phosphatase, placental type, alkaline phosphomonoesterase, ALP, EC 3.1.3.1, FLJ61142, glycerophosphatase, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1, Regan isozyme
• Host Species: Mouse
• Molecular Weight of Antigen: 70 kDa
• Quantity: 0.2 mg
• Research Discipline: Cancer, Embryonic Stem Cell Markers, Ovarian Carcinoma Cell Markers, Stem Cell Markers
• Gene ID (Entrez): 250
• Target Species: Human
• Form: Purified

For Research Use Only