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Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free, Novus Biologicals™
SDP

Catalog No. NB600540 Shop All R&D Systems Products
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NB600540 0.05 mg
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Catalog No. NB600540 Supplier Novus Biologicals Supplier No. NB600540
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Description

Mouse Monoclonal Antibody has been used in 3 publications

Alkaline Phosphatase, Tissue Non-Specific Monoclonal specifically detects Alkaline Phosphatase, Tissue Non-Specific in Human, Porcine, Feline, Rabbit samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Specifications

Antigen Alkaline Phosphatase, Tissue Non-Specific
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Classification Monoclonal
Clone TRA-2-49
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot, Flow Cytometry 5-20 ug/ml, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500
Gene Alias Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935
Gene Symbols ALPL
Host Species Mouse
Immunogen 2102Ep Human Embryonal Carcinoma Cells
Purification Method Protein A purified
Quantity 0.05 mg
Regulatory Status RUO
Research Discipline Embryonic Stem Cell Markers, Protein Phosphatase, Stem Cell Markers
Primary or Secondary Primary
Gene ID (Entrez) 249
Test Specificity Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver/ Bone/ Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells. This antibody does not cross react with other isozymes of AP.
Target Species Human, Pig, Feline, Rabbit
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1
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For Research Use Only

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