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alpha-Fetoprotein/AFP Antibody (MBS-12), Novus Biologicals™
SDP

Catalog No. NBP24501301 Shop All R&D Systems Products
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NBP24501301 0.1 mg
NBP24501300 0.02 mg
NBP24501302 0.2 mg
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Catalog No. NBP24501301 Supplier Novus Biologicals Supplier No. NBP2450130.1MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

alpha-Fetoprotein/AFP Monoclonal specifically detects alpha-Fetoprotein/AFP in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

Antigen alpha-Fetoprotein/AFP
Applications Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Classification Monoclonal
Clone MBS-12
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 1 - 2 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P02771
Gene Alias Alpha-1-fetoprotein, Alpha-fetoglobulin, alpha-fetoprotein, FETA, HP, HPAFP
Gene Symbols AFP
Host Species Mouse
Immunogen Recombinant full-length human Alpha fetoprotein
Molecular Weight of Antigen 70 kDa
Purification Method Protein A or G purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Cancer, Cancer Stem Cells, GPCR, Stem Cell Markers, Tumor Biomarkers
Primary or Secondary Primary
Gene ID (Entrez) 174
Test Specificity It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. The yolk sac and the liver produce AFP during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spinal bifida and anencephaly.
Target Species Human
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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For Research Use Only

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