Novus Biologicals™ alpha-Fetoprotein/AFP Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human alpha-Fetoprotein/AFP.

Specifications

• Assay Range: 11 pg/mL
• Assay Sensitivity: 10.94-700 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human alpha-Fetoprotein/AFP.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: Alpha-1-fetoprotein, Alpha-fetoglobulin, alpha-fetoprotein, FETA, HP, HPAFP
• Gene ID (Entrez): 174
• Gene Symbol: AFP
• Host Species: Mouse
• Kit Contents:
  Detection Antibody: 0.5 mg/mL mouse anti-alpha-Fetoprotein/AFP monoclonal antibody conjugated to horseradish-peroxidase (HRP).
  Capture Antibody: 0.1 mg/mL of mouse anti-alpha-Fetoprotein/AFP monoclonal antibody.
  Standard: Each vial contains 38 ng of recombinant alpha-Fetoprotein/AFP.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Cancer Stem Cells, Developmental Biology, GPCR, Stem Cell Markers, Tumor Biomarkers
• Storage Requirements: Storage is content dependent.
• Target: alpha-Fetoprotein/AFP
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.7 ng/mL is recommended.