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alpha-Smooth Muscle Actin Antibody (1A4/asm-1), Alexa Fluor™ 594, Novus Biologicals™
SDP

Catalog No. NP23306A594 Shop All R&D Systems Products
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NP23306A594 0.1 mL
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Catalog No. NP23306A594 Supplier Novus Biologicals Supplier No. NBP233006AF594
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Description

Mouse Monoclonal Antibody

alpha-Smooth Muscle Actin Monoclonal specifically detects alpha-Smooth Muscle Actin in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Baboon, Monkey, Rabbit, Sheep samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), Product Image.

Specifications

Antigen alpha-Smooth Muscle Actin
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone 1A4/asm-1
Conjugate Alexa Fluor 594
Dilution Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular)
Formulation 50 mM sodium borate with 0.05% sodium azide
Gene Alias AAT6, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTSA, ACTVS, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, sma, smooth muscle actin
Gene Symbols ACTA2
Host Species Mouse
Immunogen Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) is made to the N-Terminal decapeptide of Alpha smooth muscle isoform of actin and conjugated to KLH.
Purification Method Protein A purified
Quantity 0.1 mL
Research Discipline Angiogenesis, Cancer, Cardiovascular Biology, Cell Biology, Cellular Markers, Developmental Biology, Extracellular Matrix, Signal Transduction, Stem Cell Markers
Primary or Secondary Primary
Gene ID (Entrez) 59.0
Test Specificity Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Target Species Human, Mouse, Rat, Pig, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Baboon, Monkey, Rabbit, Sheep
Content And Storage Store at 4°C in the dark.
Isotype IgG2a κ
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