alpha-Smooth Muscle Actin Antibody (ACTA2/791), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24446300	Novus Biologicals NBP2444630.02MG	0.02 mg
NBP24446301	Novus Biologicals NBP2444630.1MG	0.1 mg
NBP24446302	Novus Biologicals NBP2444630.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

alpha-Smooth Muscle Actin Monoclonal specifically detects alpha-Smooth Muscle Actin in Human, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

• Antigen: alpha-Smooth Muscle Actin
• Concentration: 0.2mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Stem Cell Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: AAT6, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTVS, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, sma, smooth muscle actin
• Gene Symbols: ACTA2
• Isotype: IgG2a κ
• Purification Method: Protein A or G purified
• Test Specificity: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

• Clone: ACTA2/791
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Rat
• Gene Accession No.: P62736
• Gene ID (Entrez): 59
• Immunogen: Recombinant full-length human ACTA2 protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 42 kDa

For Research Use Only