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  6. alpha-Smooth Muscle Actin Antibody (ACTA2/791), Novus Biologicals™

alpha-Smooth Muscle Actin Antibody (ACTA2/791), Novus Biologicals™
SDP

Catalog No. NBP24446300 Shop All R&D Systems Products
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NBP24446300 0.02 mg
NBP24446301 0.1 mg
NBP24446302 0.2 mg
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Catalog No. NBP24446300 Supplier Novus Biologicals Supplier No. NBP2444630.02MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

alpha-Smooth Muscle Actin Monoclonal specifically detects alpha-Smooth Muscle Actin in Human, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

Antigen alpha-Smooth Muscle Actin
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone ACTA2/791
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P62736
Gene Alias AAT6, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTVS, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, sma, smooth muscle actin
Gene Symbols ACTA2
Host Species Mouse
Immunogen Recombinant full-length human ACTA2 protein
Molecular Weight of Antigen 42 kDa
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Stem Cell Markers
Primary or Secondary Primary
Gene ID (Entrez) 59
Test Specificity Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Target Species Human, Rat
Content And Storage Store at 4C.
Form Purified
Isotype IgG2a κ
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