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  6. alpha-Smooth Muscle Actin Mouse, Clone: 1A4/asm-1, Novus Biologicals™

alpha-Smooth Muscle Actin Mouse, Clone: 1A4/asm-1, Novus Biologicals™
SDP

Catalog No. NBP233006A Shop All R&D Systems Products
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Catalog No. Quantity
NBP233006A 0.1 mg
NBP233006F 0.02 mg
NBP233006B 0.2 mg
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Catalog No. NBP233006A Supplier Novus Biologicals Supplier No. NBP2330060.1MG
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Description

Mouse Monoclonal Antibody has been used in 23 publications

alpha-Smooth Muscle Actin Monoclonal specifically detects alpha-Smooth Muscle Actin in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Baboon, Monkey, Rabbit, Sheep samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular).

Specifications

Antigen alpha-Smooth Muscle Actin
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Classification Monoclonal
Clone 1A4/asm-1
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5-1ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 1:600
Formulation PBS with 0.02% Sodium Azide
Gene Accession No. P62736
Gene Alias 1a4 sma, 1a4 smooth muscle actin, AAT6, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTVS, alpha 2 actin, alpha SMA, alpha sma 1a4, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, sma, sma 1a4, smooth muscle actin
Gene Symbols ACTA2
Host Species Mouse
Immunogen N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH
Molecular Weight of Antigen 42 kDa
Purification Method Protein A purified
Quantity 0.1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 59
Test Specificity Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Target Species Human, Mouse, Rat, Pig, Baboon, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Monkey, Rabbit, Sheep
Content And Storage Store at 4C.
Form Purified
Isotype IgG2a κ
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