alpha-Smooth Muscle Actin Mouse, Clone: SPM332, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 4 publications

Supplier:  Novus Biologicals NBP2328080.2MG

Catalog No. NBP232808B

Description

alpha-Smooth Muscle Actin Monoclonal antibody specifically detects Antigen in Baboon, Bovine, Canine, Chicken, Feline, Goat, Guinea Pig, Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting.

Specifications

• Antigen: alpha-Smooth Muscle Actin
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: AAT6, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTVS, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, sma, smooth muscle actin
• Host Species: Mouse
• Molecular Weight of Antigen: 42 kDa
• Quantity: 0.2 mg
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Phospho Specific, Stem Cell Markers
• Gene ID (Entrez): 59
• Target Species: Human, Mouse, Rat, Pig, Baboon, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Primate, Rabbit, Sheep
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Clone: SPM332
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Gene Accession No.: P62736
• Gene Symbols: ACTA2
• Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ