Ambion™ Cells-to-CT™ Power S 1-Step Power SYBR Green Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Makes it possible to perform expression analysis directly from cultured cells without RNA purification

Manufacturer: Invitrogen A25600

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Catalog No. A25600

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    Detection Method SYBR
    For Use With (Application) 7500 Fast Dx System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio, Real-Time PCR Systems or Thermal Cyclers, StepOne System, StepOnePlus System, ViiA 7 System
    Includes Lysis Solution, 5.5mL; DNase I, 55μL; Stop Solution, 500μL; Power SYBR Green 1-Step qPCR Mix, 5mL; Power SYBR Green 1-Step RT Mix, 80μL
    Quantity 100 preps
    Sample Type Cells, RNA

    The one-step format of RT-PCR step streamlines the Cells-to- CT “sample to answer” workflow more than ever without compromising sensitivity and specificity and at extraordinary value.

    • Complete: optimized workflow includes cell lysis reagents with gDNA removal and Power SYBR Green 1-Step RT-PCR Master Mix
      Faster: 7-minute sample prep, including DNase treatment, versus 30-60 minutes for traditional RNA purification
    • Simple: 12 steps total (sample prep and real-time RT-PCR combined)
    • Robust: Perform gene expression analysis on 10 - 100,000 cells per sample; results equivalent to those from purified RNA
    • Study multiple targets per sample at an affordable price

    Accessory Product: The SYBR Green Cells-to-CT Control Kit was designed for use with SYBR Green Cells-to-CT products. This kit contains an endogenous control primer set (ACTB) to normalize for sample loading variability and an artifical XenoRNA control and corresponding primer set to monitor the effects of PCR inhibition.

    Gene Expression Analysis & Genotyping, Real Time PCR-Based Gene Expression Profiling

  • Description & Specifications

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