Aminopeptidase N/CD13 Antibody (APN/514) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2478920.1MG

Catalog No. NBP247892A

Description

Aminopeptidase N/CD13 Monoclonal specifically detects Aminopeptidase N/CD13 in Human samples. It is validated for ELISA.

Specifications

• Antigen: Aminopeptidase N/CD13
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Gene Alias: alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, aminopeptidase N, AP-M, AP-N, CD13 antigen, CD13APN, EC 3.4.11, EC 3.4.11.2, gp150, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, P150, PEPNhAPN
• Host Species: Mouse
• Molecular Weight of Antigen: 150 kDa
• Quantity: 0.1 mg
• Research Discipline: Angiogenesis, Cellular Markers, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Myeloid derived Suppressor Cell, Stem Cell Markers
• Gene ID (Entrez): 290
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: APN/514
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: ANPEP
• Immunogen: Recombinant human CD13 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes an extracellular epitope of an integral membrane glycoprotein of 150kDa, identified as CD13. This antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. It is absent from normal lymphocytes, platelets and erythrocytes. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves as a receptor for human coronaviruses (HCV). The lineage-restricted pattern of expression of CD13 within the hemopoietic compartment suggests that it may be important in myeloid cell differentiation.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ