Amphiregulin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P322BT25	Invitrogen™ 500P322BT25UG	25 μg
500P322BT50	Invitrogen™ 500P322BT50UG	50 μg
50-271-8509	Invitrogen™ 500P322BT500UG	500 μg

Description

AA Sequence of recombinant protein: SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100 μL/well), a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Amphiregulin (500-P322) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human Amphiregulin. Western Blot: To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P322BT-1MG will be provided as 2 x 500 μg

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

Specifications

• Antigen: Amphiregulin
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P15514
• Gene ID (Entrez): 374
• Immunogen: E.coli-derived Recombinant Human Amphiregulin.
• Purification Method: Antigen affinity chromatography
• Gene: AREG

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: amphiregulin; amphiregulin B; AR; AREG; AREGB; colorectum cell-derived growth factor; CRDGF; Mcub; MGC13647; schwannoma-derived growth factor; SDGF
• Gene Symbols: AREG
• Primary or Secondary: Primary
• Content And Storage: -20°C