Invitrogen™ Ankyrin 1 Polyclonal Antibody

Catalog No.	Quantity
PIPA5143887	100 μg

Catalog No. PIPA5143887 Supplier Invitrogen™ Supplier No. PA5143887

Description

Rabbit Polyclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human HEL whole cell, human K562 whole cell. IHC: human colon cancer tissue, mouse cerebellum tissue, rat cerebellum tissue. ICC/IF: A549 cell. Flow: HEL cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

Specifications

• Antigen: Ankyrin 1
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and no preservative
• Gene: ANK1
• Gene Accession No.: P16157, Q02357
• Gene Alias: ANK; ANK1; ANK-1; ANK-1; erythrocyte skeletal protein; ankyrin 1; ankyrin 1, erythrocytic; ankyrin 1, erythroid; ankyrin-1; Ankyrin-R; erythrocyte ankyrin; nb; normoblastic anemia; pale; rCG_43073; SPH1; SPH2
• Gene Symbols: ANK1
• Host Species: Rabbit
• Immunogen: E.coli-derived human Ankyrin erythroid/ANK/ANK1 recombinant protein (Position: N1300-Q1844).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11733, 286, 306570
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG