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Apaf-1 Mouse anti-Human, Unlabeled, Clone: 24, BD

Catalog No. BDB611364 Shop All BD Biosciences Products
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BDB611364 50 μg
BDB611365 150 μg
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Catalog No. BDB611364 Supplier BD Biosciences Supplier No. 611364

Item Discontinued This item has been discontinued by the supplier. Please to view product availability in your area.

Description

Mouse Monoclonal Antibody

The process of apoptosis requires the activation of aspartate-specific cystenine proteases in the caspase family. Group I caspases (1,4,5) cleave at (W/L)EHD tetrapeptide motifs, while group II caspases (2,3,7) cleave the DEXD tetrapeptide motif. Group III caspases (6,8,9) are activators of other caspases via cleavage of (I/V)EXD tetrapeptide sequences. Apoptotic protease-activating factor-1 (Apaf-1), cytochrome c, and dATP activate caspase-9, which in turn, initiates the post-mitochondrial-mediated caspase cascade that includes caspase-2, 3, 6, 7, 8 and 10. Apaf-1 is a soluble protein with a short N-terminal caspase recruitment domain (CARD), a central CED-4 homology domain, and 12 WD-40 repeats that may be involved in protein-protein interactions. During apoptosis, a large (700kDa) aposome complex containing Apaf-1, cytochrome c, caspase-3, 7, and 9, and a smaller (200-300kDa) microaposome complex containing caspase-3 and 7 exhibit higher cleavage activity than “free” caspase heterotetramers. Thus Apaf-1 is a component of the large aposome complex, which functions in caspase activation leading to caspase-dependent proteolytic events and apoptosis.

Immunofluorescence, Western Blotting

TRUSTED_SUSTAINABILITY

Specifications

Antigen Apaf-1
Applications Western Blot
Classification Monoclonal
Clone 24
Concentration 250μg/mL
Conjugate Unconjugated
Description Apoptotic Protease Activating Factor-1
Formulation Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human Apaf-1 aa. 252-445
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Research Discipline Cell Biology
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at -20°C.
Isotype IgG1
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For Research Use Only.

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