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Apaf-1 Mouse anti-Human, Unlabeled, Clone: 24, BD

Mouse Monoclonal Antibody

$213.00

Specifications

Antigen Apaf-1
Isotype IgG1
Immunogen Human Apaf-1 aa. 252-445
Formulation Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Conjugate Unconjugated
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
BDB611364
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BD Biosciences
611364
50μg
Each for $213.00
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BDB611365
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BD Biosciences
611365
150μg This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. N/A
Description

Description

The process of apoptosis requires the activation of aspartate-specific cystenine proteases in the caspase family. Group I caspases (1,4,5) cleave at (W/L)EHD tetrapeptide motifs, while group II caspases (2,3,7) cleave the DEXD tetrapeptide motif. Group III caspases (6,8,9) are activators of other caspases via cleavage of (I/V)EXD tetrapeptide sequences. Apoptotic protease-activating factor-1 (Apaf-1), cytochrome c, and dATP activate caspase-9, which in turn, initiates the post-mitochondrial-mediated caspase cascade that includes caspase-2, 3, 6, 7, 8 and 10. Apaf-1 is a soluble protein with a short N-terminal caspase recruitment domain (CARD), a central CED-4 homology domain, and 12 WD-40 repeats that may be involved in protein-protein interactions. During apoptosis, a large (700kDa) aposome complex containing Apaf-1, cytochrome c, caspase-3, 7, and 9, and a smaller (200-300kDa) microaposome complex containing caspase-3 and 7 exhibit higher cleavage activity than “free” caspase heterotetramers. Thus Apaf-1 is a component of the large aposome complex, which functions in caspase activation leading to caspase-dependent proteolytic events and apoptosis.

Immunofluorescence, Western Blotting

Specifications

Specifications

Apaf-1
Human Apaf-1 aa. 252-445
Unconjugated
Cell Biology
Mouse
Affinity Purified
Monoclonal
Apoptotic Protease Activating Factor-1
IgG1
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Primary
Western Blot
RUO
24
250μg/mL
Store undiluted at -20°C.
SDS
Product Certifications

For Research Use Only.

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