Calcineurin Mouse, Unlabeled, Clone: 29, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 610259

Catalog No. BDB610259

Description

Calcineurin is a Ca²⁺/calmodulin-dependent protein phosphatase. The active enzyme is a heterodimer of a large calmodulin-binding catalytic subunit A (61kDa) and a smaller Ca²⁺ binding subunit B (19kDa). Regions corresponding to the calmodulin-binding site, an autoinhibitory domain, and a putative subunit B binding site have been identified within the large subunit A. The activity of calcineurin is sensitive to immunosuppressants such as cyclosporin A (CsA) and tacrolimus (FK506). The study of FK506-mediated inhibition of nitric oxide formation has revealed that nitric oxide synthase (NOS) is a calcineurin substrate. Calcineurin dephosphorylates NOS and enhances its catalytic activity. Therefore, Calcineurin is an essential mediator for efficient T cell antigen receptor (TCR)-mediated T cell activation.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: Calcineurin
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Chicken, Frog, Human, Mouse, Rat
• Isotype: IgG2a

• Applications: Western Blot
• Clone: 29
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.