CD3 Mouse anti-Human, BB515, Clone: UCHT1, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB564466	BD Biosciences 564466	25 Tests
BDB564465	BD Biosciences 564465	100 Tests

Description

The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .

The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

• Antigen: CD3
• Applications: Flow Cytometry
• Conjugate: Brilliant Blue 515 (BB515)
• Regulatory Status: RUO
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: UCHT1
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon

For Research Use Only.