CD5 Rat anti-Mouse, FITC, Clone: 53-7.3, BD

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB553020	BD Biosciences 553020	0.1 mg
BDB553021	BD Biosciences 553021	0.5 mg

Description

The 53-7.3 clone has been reported to react with a monomorphic epitope of CD5, a member of the scavenger receptor cysteine-rich protein superfamily and the major ligand of CD72, found on thymocytes, T lymphocytes, thymic NK-T cells, and a subset of B lymphocytes, but not on NK cells or splenic NK-T cells. The level of surface CD5 expression is developmentally regulated in the thymus, starting with low levels on CD4-CD8- thymocytes and increasing as they mature to CD4+CD8+ then CD4+CD8- or CD4-CD8+ thymocytes. Relatively high levels are maintained on peripheral T lymphocytes. The level of CD5 antigen detected on T helper cells has been reported to be somewhat higher than that on T cytotoxic/suppressor and B cells. Few, if any, intestinal intraepithelial lymphocytes bearing the γδ T-cell receptor express CD5. Phenotypic, anatomical, functional, developmental, and pathogenic characteristics of peripheral CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. The frequency of these CD5+ B cells has been reported to show strain-dependent variation. An additional population of CD5+ B lymphocytes resides in the thymus, where it matures from intrathymic B-cell progenitors. It has been proposed that CD5 is a costimulatory molecule which mediates interactions of cells in the immune system and negatively regulates signal transduction mediated by the T-cell receptor and B-cell receptor. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Rat
Clone: 53-7.3
Isotype: IgG2a κ
Species Reactivity: Mouse
Immunogen: Mouse Thymus / Spleen

Flow Cytometry

Specifications

• Antigen: CD5
• Concentration: 0.5mg/mL
• Classification: Monoclonal
• Host Species: Rat
• Research Discipline: Immunology
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG2a κ
• Purification Method: Affinity Purified
• Description: Lymphocyte antigen 1; Cd5; Ly-12; Ly-A; Lyt-1

• Clone: 53-7.3
• Applications: Flow Cytometry
• Conjugate: FITC
• Regulatory Status: RUO
• Target Species: Mouse
• Immunogen: Mouse Thymus / Spleen
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.