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CD8 Mouse anti-Human, Brilliant Violet 650, Clone: RPA-T8, BD
Mouse Monoclonal Antibody
$214.00 - $430.00
Specifications
Antigen | CD8 |
---|---|
Clone | RPA-T8 |
Applications | Flow Cytometry |
Classification | Monoclonal |
Conjugate | Brilliant Violet 650 |
Description
The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α) . CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8 α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
CD8 | |
Flow Cytometry | |
Brilliant Violet 650 | |
RUO | |
Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
IgG1 κ | |
Affinity Purified | |
CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32 |
RPA-T8 | |
Monoclonal | |
Mouse | |
Baboon, Cynomolgus, Human, Rhesus Monkey | |
Human CD8a | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
For Research Use Only.
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