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CD8 Mouse anti-Human, Brilliant Violet 650, Clone: RPA-T8, BD

Mouse Monoclonal Antibody

$196.00 - $396.00

Specifications

Antigen CD8
Isotype IgG1, κ
Immunogen Human CD8a
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Conjugate Brilliant Violet 650
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
BDB563822
View Documents Promotion Details
BD Biosciences
563822
25 Tests
Each for $196.00
Add to cart
 
BDB563821
View Documents Promotion Details
BD Biosciences
563821
100 Tests
Each for $396.00
Add to cart
 
Description

Description

The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α) . CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8 α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

Specifications

CD8
Human CD8a
Brilliant Violet 650
Flow Cytometry
RUO
RPA-T8
CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
IgG1, κ
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Primary
Mouse
Affinity Purified
Monoclonal
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
SDS
Product Certifications

For Research Use Only.

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