CD80 Mouse anti-Human, BB515, Clone: L307.4, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB565009	BD Biosciences 565009	25 Tests
BDB565008	BD Biosciences 565008	100 Tests

Description

The L307.4 monoclonal antibody specifically binds to B7/BB1, a 60 kDa transmembrane glycoprotein that was clustered as CD80 in the Fifth International Workshop on Human Leukocyte Differentiation Antigens. CD80, a member of the Ig supergene family, is expressed on activated B cells, T cells, macrophages, and dendritic cells. It is the ligand for two molecules expressed on T cells, CD28 and CD152 (CTLA-4). CD80 is also expressed on activated CD4-positive and CD8-positive T cells, appearing late after activation suggesting that activated T cells may be capable of autocrine costimulation via the CD28 activation pathway. The binding of CD28 by anti-CD28 or by CD80 results in T-cell activation and a signal for IL-2 production.

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

• Antigen: CD80
• Applications: Flow Cytometry, Immunohistochemistry, Western Blot
• Conjugate: Brilliant Blue 515 (BB515)
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: B7.1; B7-1; Activation B7-1 antigen; B7; BB1; CD28LG; CD28LG1; LAB7

• Clone: L307.4
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Immunogen: Human B7-transfected L cells
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.