FoxP3 Mouse anti-Human, V450, Clone: 259D/C7, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB560460	BD Biosciences 560460	30 Tests
BDB560459	BD Biosciences 560459	120 Tests

Description

The 259D/C7 antibody reacts with the human FoxP3 transcription factor, a member of the forkhead or winged helix family of transcription factors. The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+ regulatory T cells and represents a specific marker for these cells. Flow-cytometric analysis has shown that FoxP3 is expressed by the majority of CD4+CD25+high T cells in peripheral blood while less than half of CD4+CD25int cell population are FoxP3 positive. Approximately 5-10% of peripheral CD4+ cells are CD4+CD25+ T regulatory cells. T regulatory cells are thought to play a critical role in the control of T cell mediated
autoimmunity by suppressing the proliferation and cytokine production of other T cells. To support this hypothesis, it has been found that FOXP3 is mutated in scurfy (sf) mice. The 259D/C7 antibody reacts with all currently identified isoforms of human FoxP3 and is cross-reactive with Cynomolgus, Rhesus and Baboon. The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Host Species: Mouse
Clone: 259D/C7
Isotype: IgG1
Species Reactivity: Human
Immunogen: FoxP3 recombinant protein

Intracellular Staining

Specifications

• Antigen: FoxP3
• Applications: Flow Cytometry
• Conjugate: Violet Fluor 450 (V450)
• Regulatory Status: RUO
• Target Species: Baboon, Cynomolgus, Human, Rhesus Monkey
• Immunogen: FoxP3 recombinant protein
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: 259D/C7
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Immunology
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Isotype: IgG1
• Purification Method: Affinity Purified
• Description: Scurfin, IPEX, JM2

For Research Use Only.