LC3A Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 265 publications

Supplier:  Novus Biologicals NB1002331SS

Catalog No. NB1002331SS

Description

LC3A Polyclonal specifically detects LC3A in Human, Mouse, Rat, Amphibian, Canine, Fish, Plant, Zebrafish samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Southern Blot, Immunohistochemistry Whole-Mount.

Specifications

• Antigen: LC3A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% Sodium Azide
• Gene Alias: ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, lc3-i, ii, MAP1A/1B light chain 3 A, MAP1ALC3MAP1A/MAP1B LC3 A, MAP1BLC3MAP1A/MAP1B light chain 3 A, microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody detects both LC3A and LC3B.
• Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

• Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization (ISH)
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 2.0 ug/ml, Simple Western 1:50, Chromatin Immunoprecipitation reported in scientific literature (PMID 33035707), Flow Cytometry reported in scientific literature (PMID 24419333), ELISA reported in scientific literature (PMID 20930550), Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:100-1:300. Use reported in scientific literature (PMID 21545732), Immunoprecipitation 20 ug/ 500 ug of lysate, Immunohistochemistry-Paraffin 1:200-1:400. Use reported in scientific literature (PMID 26571030), Immunohistochemistry-Frozen, Immunoblotting reported in scientific literature (PMID 28253371), Southern Blot, Immunohistochemistry (Whole-Mount) reported in scientific literature (PMID 31783118), Chromatin Immunoprecipitation (ChIP)
• Gene Accession No.: Q9GZQ8
• Gene Symbols: MAP1LC3A
• Immunogen: A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492].
• Quantity: 0.025 mL
• Research Discipline: Autophagy, Cancer, Cellular Markers, Cytoskeleton Markers, Hypoxia, Macroautophagy, Signal Transduction
• Gene ID (Entrez): 84557
• Target Species: Human, Mouse, Rat, Amphibian, Canine, Fish, Plant, Zebrafish
• Isotype: IgG

For Research Use Only