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CD25 Rat anti-Mouse, PE-CF594, Clone: PC61, BD

Rat Monoclonal Antibody

$135.00 - $315.00

Specifications

Antigen CD25
Isotype IgG1, λ
Immunogen IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Conjugate PE-CF594
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
BDB562695
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BD Biosciences
562695
25μg
Each for $135.00
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BDB562694
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BD Biosciences
562694
0.1mg
Each for $315.00
Add to cart
 
Description

Description

The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor α chain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No. 557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).

Specifications

Specifications

CD25
IL-2-dependent cytolytic mouse T-cell clone B6.1
PE-CF594
Flow Cytometry
RUO
PC61
0.2mg/mL
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
IgG1, λ
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Primary
Rat
Affinity Purified
Monoclonal
Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55
SDS

For Research Use Only.

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