Invitrogen™ Phospho-CaMKII alpha/beta (Thr286) Polyclonal Antibody

Catalog No.	Quantity
PA14614	100 μL

Catalog No. PA14614 Supplier Invitrogen™ Supplier No. PA14614

Description

Rabbit Polyclonal Antibody

This antibody is predicted to react with human, mouse and Xenopus based on 100% sequence homology. This antibody is specific for the ~50 kDa alpha-CaM Kinase II subunit and the ~60 kDa beta-CaM Kinase II subunit phosphorylated at Thr286 in Western blots of rat brain lysates. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular response to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduces LTP and learning (Elgersma et al., 2002).

Specifications

• Antigen: Phospho-CaMKII alpha/beta (Thr286)
• Applications: Immunohistochemistry, Western Blot
• Classification: Polyclonal
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene: Camk2b
• Gene Accession No.: P08413, P11275, P11798, P28652, Q13554, Q9UQM7
• Gene Alias: alpha CaM kinase II; alpha-CaMKII; Ca++/calmodulin-dependent protein kinase II beta subunit; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase II, beta; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II beta chain; calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit beta; caM kinase II subunit alpha; CaM kinase II subunit beta; CaMK II; Camk2a; Camk2b; Camk2d; CaMKII; caMK-II subunit alpha; caMK-II subunit beta; caM-kinase II alpha chain; caM-kinase II beta chain; Ck2b; mKIAA0968; PK2CDD; PKCCD; R74975
• Gene Symbols: Camk2a, Camk2b
• Host Species: Rabbit
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CAMK2A/B conjugated to KLH.
• Purification Method: Affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100341507, 100342451, 12322, 12323, 24245, 25400, 815, 816
• Target Species: Human, Mouse, Rat, Rabbit
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG