PKA (RIIα) Mouse, Unlabeled, Clone: 40, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB612242	BD Biosciences 612242	50 μg
BDB612243	BD Biosciences 612243	150 μg

Description

cAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic(C) and regulatory (R). Four regulatory subunits have been identified: RIα, RIβ, RIIα, and RIIβ.These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Cα, Cβ, or Cγ). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Rα isoforms are ubiquitously expressed, the Rβ isoforms are predominant in nervous and adipose tissues. Expression of the RIα subunit is modulated during muscle and adipocyte differentiation in vitro.

Immunofluorescence, Immunoprecipitation, Western Blotting

Specifications

• Antigen: PKA (RIIα)
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgG1
• Purification Method: Affinity Purified

• Clone: 40
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Canine, Human, Mouse, Rat
• Immunogen: Human PKA[RIIα] aa. 1-404
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.