PPAR alpha Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1822A
PA1-822A detects peroxisome proliferator activated receptor (PPAR) alpha from mouse samples. PA1-822A has been successfully used in Western blot, immunoprecipitation and immunofluoresence procedures. By Western blot, this antibody detects an ~52 kDa protein representing PPAR alpha from 3T3-L1 cell lysate. Immunofluoresence staining is observed predomitly in the nucleus as well as the cytoplasm. PA1-822A immunogen is a synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha. A single amino acid substitution (I8 to L8) exists between the mouse and human protein. This immunizing peptide (Cat.No. PEP-025) is available for use in neutralization and control experiments.Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR’s). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR’s are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR’s can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
|Synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha.|
|Antigen affinity chromatography|
|Immunofluorescence, Immunoprecipitation, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Peroxisome Proliferator Activated Receptor Alpha|
|-20° C, Avoid Freeze/Thaw Cycles|
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