Tim23 Mouse, Unlabeled, Clone: 32, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB611222	BD Biosciences 611222	50 μg
BDB611223	BD Biosciences 611223	150 μg

Description

Mitochondria, the site of cellular energy production, must import all proteins necessary for their function. Import is mediated by two mechanisms: the translocase of the outer membrane (Tom) and the translocase of the inner membrane (Tim). Tim23 and Tim17 are integral membrane proteins that associate to form the import channel for mitochondrial preproteins that contain N-terminal hydrophilic sequences. They also associate with Tim44, an adaptor for the membrane binding of mtHsp70, a matrix heat shock protein, which drives the import of the
processed preprotein. The N-terminal intermembrane space domain of Tim23 contains a leucine zipper motif and mediates the formation of a Tim23 dimer. As an imported protein passes through the TOM machinery, its N-terminal matrix targeting sequence interacts with the Tim23 dimer. This induces the dissociation of the dimer and initiation of inner membrane translocation of the presequence. In addition to its 9kDa N-terminal hydrophilic segment, Tim23 contains a 14kDa hydrophobic domain with four predicted membrane spans. Thus, Tim23 is an important integral membrane component of the mitochondrial protein translocation machinery.

Host Species: Mouse
Clone: 32
Isotype: IgG2a
Species Reactivity: Mouse
Immunogen: Rat Tim23 aa. 5-126
Formula Weight [Chemical]: 23kDa

Immunofluorescence, Western Blotting

Specifications

• Antigen: Tim23
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgG2a
• Purification Method: Affinity Purified

• Clone: 32
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat
• Immunogen: Rat Tim23 aa. 5-126
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.