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Invitrogen™ APC Monoclonal Antibody (eBioAPC-6A2), Biotin, eBioscience™

Mouse Monoclonal Antibody

$152.00

Specifications

Antigen APC
Clone eBioAPC-6A2
Concentration 0.5 mg/mL
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Applications Flow Cytometry
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
13-417-082
Encompass_Preferred
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Invitrogen™
13417082
100 μg
Each of 1 for $152.00
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Description

Description

Description: The eBioAPC-6A2 monoclonal antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE). When using eBioAPC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody. Therefore no interference or quenching of the APC fluorochrome is observed. The eBioAPC-6A2 can be used for separation of cells labeled with APC-conjugated antibodies or for staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary APC-conjugated antibody and the application tested. Applications Reported: This eBioAPC-6A2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioAPC-6A2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained with an APC-conjugated antibody. This can be used at less than or equal to 0.015 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Anti-APC (Allophycocyanin) antibodies can be used to bind APC-conjugated primary antibodies without decreasing fluorescent intensity of the APC fluorochrome. This method is used for separation of cells labeled with APC-conjugated antibodies.
Specifications

Specifications

APC
0.5 mg/mL
Flow Cytometry
Biotin
Mouse
RUO
PBS with 0.09% sodium azide; pH 7.2
Primary
Affinity Chromatography
eBioAPC-6A2
4°C, store in dark, DO NOT FREEZE!
Monoclonal
Liquid
IgG1
Tag
APC
Antibody
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Documents

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