Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Catalog No.	No. of Reactions
43-520-42	250 Reactions
43-660-72	500 Reactions
43-678-46	5000 Reactions
43-660-73	1250 Reactions
43-641-03	2500 Reactions

Catalog No. 43-520-42 Supplier Applied Biosystems™ Supplier No. 4352042

Description

Requires

Template and primers

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

• Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
• The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
• A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
• Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
• TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
• Compatible with TaqMan Gene Expression assays
• Store at 2°C to 8°C

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

• Concentration: 2X
• Content And Storage: TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains two tubes of 1.25 mL each sufficient for 250 20-μL PCR reactions. Store at 2-8°C
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Detection Method: Primer-probe
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: AmpliTaq DNA Polymerase
• Reaction Speed: Fast
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: AmpErase, TaqMan
• Product Type: Fast Universal PCR Master Mix
• Quantity: 250 reactions
• Shipping Condition: Wet Ice
• Sufficient For: 250 Reactions
• For Use With (Application): Gene Expression, Genotyping, miRNA analysis
• Fidelity (vs. Taq): 2 X
• No. of Reactions: 250 Reactions
• Sample Type: DNA (Genomic), cDNA
• Volume: 20 μL

Frequently Asked Questions (FAQs)

Can SNP assays be run using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

It may be technically feasible, but we have not fully validated TaqMan SNP Genotyping assays on the Applied Biosystems 7500 Fast System and therefore cannot recommend it.

What real-time PCR applications can I perform using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

You can perform experiments for Absolute Quantitation and Relative Quantitation with fast cycling. However, Allelic Discrimination (SNP) assays and plus/minus assays using internal positive controls cannot be run with fast reagents and protocols.

For Research Use Only. Not for use in diagnostic procedures.