Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Catalog No.	No. of Reactions
43-641-03	2500 Reactions
43-660-72	500 Reactions
43-678-46	5000 Reactions
43-520-42	250 Reactions
43-660-73	1250 Reactions

Catalog No. 43-641-03 Supplier Applied Biosystems™ Supplier No. 4364103

Description

Requires

Template and primers

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

• Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
• The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
• A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
• Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
• TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
• Compatible with TaqMan Gene Expression assays
• Store at 2°C to 8°C

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

• Concentration: 2X
• Content And Storage: TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains 10 packs of 2 tubes (1.25 ml each), sufficient for 2500 PCR reactions (20 μl each).
  
  Store at 2°C to 8°C.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
  
  
• Detection Method: Primer-probe
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: AmpliTaq DNA Polymerase
• Reaction Speed: Fast
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: AmpErase, TaqMan
• Product Type: Fast Universal PCR Master Mix
• Quantity: 2,500 reactions
• Sufficient For: 2500 Reactions
• For Use With (Application): Gene Expression, Genotyping, miRNA analysis
• Fidelity (vs. Taq): 2 X
• No. of Reactions: 2500 Reactions
• Sample Type: DNA (Genomic), cDNA
• Volume: 20 μL

Frequently Asked Questions (FAQs)

What is the lowest copy number that can be used with the TaqMan Fast Universal PCR Master Mix (P/N 4352042)?

The fast master mix has demonstrated detection down to 10 copies of the RNase P gene with genomic DNA. Of course, detection limits may vary depending on factors such as assay design and sample preparation.

Which TaqMan assay types can be used when using the with TaqMan Fast Universal PCR Master Mix (P/N 4352042)?

The following research assays are supported using the TaqMan Fast Universal and/or Advanced Fast PCR Master Mix: TaqMan Gene Expression Assays, Custom TaqMan Gene Expression Assays, Custom primers and probes designed from Primer Express software-designed quantitative assays.

Are there any recommendations for running primer-limited VIC dye-labeled  TaqMan Endogenous Controls using the Fast Universal PCR Master Mix such as Cat. No. 4352042?

For using primer-limited VIC dye-labeled TaqMan Endogenous Controls in a single-plex reaction, we recommend starting with an annealing/extension time of 30 sec and extension temperature of 62 ºC. For optimal performance, the recommendation is to use the TaqMan Fast Advanced Master Mix for such applications.

Are there any recommendations for running multiplex assays using the Fast Universal PCR Master Mix such as Cat. No. 4352042?

Multiplex assays applications are complex with variable performance results across different assays. Our recommendation is to use the TaqMan Fast Advanced Master Mix for such applications.

Can SNP assays be run using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

It may be technically feasible, but we have not fully validated TaqMan SNP Genotyping assays on the Applied Biosystems 7500 Fast System and therefore cannot recommend it.

What real-time PCR applications can I perform using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

You can perform experiments for Absolute Quantitation and Relative Quantitation with fast cycling. However, Allelic Discrimination (SNP) assays and plus/minus assays using internal positive controls cannot be run with fast reagents and protocols.

For Research Use Only. Not for use in diagnostic procedures.