Applied Biosystems™ MeltDoctor™ HRM Master Mix

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

Supplier:  Applied Biosystems™ 4415450

Catalog No. 44-154-50

Includes: Provided in a convenient 2X mix, containing: AmpliTaq Gold™ 360 DNA Polymerase, MeltDoctor HRM Dye, a dNTP blend including dUTP, Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results

Description

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications.

• Low background fluorescence
• High brightness in the presence of double-stranded DNA
• Minimal temperature shift of DNA melting due to dye binding
• Thermal stability to tolerate PCR cycling conditions
• No inhibition of polymerase activity, resulting in high PCR efficiency
• A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

Perform Mutation Scanning:

• HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis
• As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE)

Advantages of HRM for mutation scanning

• Low reagent consumption, with little waste: HRM requires only a 20μL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
• Simple, fast workflow: no additional instrumentation is required after PCR amplification;A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
• Fast optimization: unlike DHPLC, thermal optimization is not required
• Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction
  Quality Components
  
  • Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
  • AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing non-specific product formation and enabling reactions to be set up at room temperature
  • MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

  Order Info

  Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice

Specifications

• Content And Storage: Components of the MeltDoctor™ HRM Master Mix, provided in a convenient 2X mix, include: AmpliTaq Gold™ 360 DNA Polymerase, MeltDoctor™ HRM Dye, a dNTP blend including dUTP, Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results.
  
  Store at -15 °C to -25 °C
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Form: Liquid
• PCR Method: qPCR
• Reaction Speed: Fast
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
• Label or Dye: High Resolution Melting (HRM), SYTO 9
• Product Type: HRM Master Mix
• For Use With (Application): Genotyping
• Sample Type: DNA (Genomic), cDNA

• Detection Method: HRM
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq Gold 360 DNA Polymerase
• Technique: HRM (High Resolution Melting) Analysis, Mutation Scanning
• Genotyping Target: SNPs (Unknown or Numerous), Epigenetic Targets
• Product Line: MeltDoctor™
• Quantity: 10 x 5 mL
• No. of Reactions: 5000 Reactions
• Volume: 50 mL

For Research Use Only. Not for use in diagnostic procedures.