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Applied Biosystems™ Fast SYBR™ Green Master Mix Non-distribution product as customer accommodation.

Designed for speed, provides a fast, reliable and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range or PCR efficiency.

Manufacturer:  Applied Biosystems™ 4385614

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Catalog No. 43-856-14


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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Requires : Template and primers

Specifications

Description

  • Fast - Real-time PCR results in as fast as 35 minutes
  • Sensitive - Detect very low copies of target
  • Specific - Minimize primer-dimer and non-specific amplification
  • Reproducible - Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix
  • AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
  • SYBR™ Green I dye to enable detection of double-stranded DNA
  • Deoxynucleotides (dNTPs) to help maintain optimal PCR results
  • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
  • Passive internal reference based on proprietary ROX™ dye to enable increased precision
Description & Specifications

Specifications

Purity or Quality Grade: UP (Ultra Pure)
The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1 and optimized buffer components. 1 x 50mL vial sufficient for 5000 reactions of 20μL each.
Store at 2° to 8°C.
Detection Method: SYBR; PCR Method: qPCR
cDNA, DNA (Genomic)
Real time PCR (qPCR), real time PCR-based gene expression profiling
1 x 50mL
Primer-Probe Compatability: Unlabeled Oligos
7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Fast
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