TaqMan™ Reverse Transcription Reagents Non-distribution product as customer accommodation.

For use with reverse transcriptase-PCR (RT-PCR)

Manufacturer: Invitrogen 4304134

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Catalog No. 43-041-34

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    Specifications

    For Use With (Application) Real Time-PCR
    Format Separate Components
    Includes MultiScribe Reverse Transcriptase (100μL at 50U/μL)
    Quantity 2000 reactions
    Sample Type RNA
    Storage Requirements Store at -20deg.C
    Temperature (English) Operating 107.6deg.F
    Temperature (Metric) Operating 42deg.C

    Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

    • Contain MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
    • Random hexamers and oligo (dT)16 included for flexibility
    • Individual components provide versatility in assay set-up
      ·Compatible with one-step or two-step RT-PCR reactions
    • Optimal reaction temperature: 42°C
    • Final product size: 5kb or less

    Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems. Built-in normalization The TaqMan reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

    Applications:

    PCR & Real-Time PCR, Reverse Transcription

  • Description & Specifications

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