Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)₁₆, and reverse primers are included for cDNA synthesis flexibility.

Supplier:  Applied Biosystems™ 4304134

Catalog No. 43-041-34

Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)

Description

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)₁₆, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA. The reagents in this kit facilitate two-step RT-PCR.

Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents
• Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)₁₆ included for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)₁₆, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

Specifications

• Content And Storage:

  Includes 10 units of Cat. No. N8080234. Each unit contains:
  
  • MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)
  • RNase Inhibitor, 100 μL (20 U/μL)
  • dNTP Mixture, 1 mL (2.5 mM each dNTP)
  • Oligo (dT)₁₆, 100 μL (50 μM)
  • Random Hexamers, 100 μL (50 μM)
  • 10X RT Buffer, 1.5 mL
  • MgCl₂ Solution, 1.5 mL
  
  Store at –15 to –25°C.
  
  

• Technique: Reverse Transcription
• Reverse Transcriptase: MultiScribe
• Shipping Condition: Dry Ice
• Concentration: 50 U/μL
• No. of Reactions: 2,000 Reactions
• Reagent Type: Reverse Transcription
• Starting Material: RNA

• Format: Kit
• Optimal Reaction Temperature: 42°C
• Ribonuclease H Activity: Yes
• For Use With (Application): Real Time PCR (qPCR)
• Final Product Type: First-Strand cDNA
• Reaction Format: Separate components
• Size (Final Product): Up to 5 kb

For Research Use Only. Not for use in diagnostic procedures.