Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Supplier:  Applied Biosystems™ 4305719

Catalog No. 43-057-19

Description

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time.

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time.

• Contains AmpliTaq Gold DNA Polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq DNA Polymerase
• Includes a passive internal reference based on proprietary ROX dye, for superb precision on Applied Biosystems real-time PCR instruments
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• AmpErase UNG protects against subsequent re-amplification from PCR products containing dU to minimize carry-over contamination
• Rapid assay development guidelines are provided to minimize optimization time

Alternative product: Try TaqMan Fast Advanced Master Mix (Cat. No. 4444963), our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

• Content And Storage: Supplied at 2X concentration. The mix contains AmpliTaq Gold™ DNA Polymerase, Uracil-DNA Glycosylase, dTNPs with dUTP, Passive Reference 1 and optimized buffer components. This pack contains 10 boxes with one 5 mL tube each, sufficient for 2000 reactions at the 50 μL total volume. Store at 2°C to 8°C in the dark.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq Gold DNA Polymerase
• For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Universal PCR Master Mix
• For Use With (Application): Gene Expression, Genotyping, miRNA analysis
• Volume: 50 mL

• Detection Method: Primer-probe
• PCR Method: qPCR
• Reaction Speed: Standard
• Kit Type: 10 Pack
• Product Line: TaqMan™
• Quantity: 10 x 5 mL
• Sample Type: DNA (Genomic), cDNA

For Research Use Only. Not for use in diagnostic procedures.