Astrocytomas Antibody (J1-31), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB019477	Novus Biologicals NBP2298200.025ML	0.025 mL
NBP229820	Novus Biologicals NBP229820	0.1 mL

Description

Astrocytomas Monoclonal specifically detects Astrocytomas in Human, Rat samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Astrocytomas
• Dilution: Western Blot 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
• Classification: Monoclonal
• Form: Ascites
• Regulatory Status: RUO
• Formulation: Ascites with No Preservative
• Immunogen: Human cerebral white matter plaque materials from a multiple sclerosis patient.
• Primary or Secondary: Primary
• Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

• Clone: J1-31
• Applications: Immunocytochemistry, Western Blot, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Rat
• Gene Alias: Astrocytoma Marker, Astrocytomas Marker, Low Grade Astrocytoma Marker
• Isotype: IgM
• Purification Method: Unpurified
• Test Specificity: The antibody recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS).The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue. The antibody is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed). Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP (Malhotra, SK (1989) J Neurosci Res. 22(1):36-49).

For Research Use Only