Novus Biologicals™ Axl Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse Axl.

Specifications

• Assay Range: 12.5 pg/mL
• Assay Sensitivity: 12.5-800 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse Axl.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: AXL oncogene, AXL receptor tyrosine kinase, AXL transforming sequence/gene, EC 2.7.10, EC 2.7.10.1, JTK11, tyrosine-protein kinase receptor UFO, UFOoncogene AXL
• Gene ID (Entrez): 558
• Gene Symbol: AXL
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Axl monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of rabbit anti-Mouse Axl monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 70 ng of recombinant Mouse Axl.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cell Cycle and Replication, Phospho Specific
• Storage Requirements: Storage is content dependent.
• Target: Axl
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/mL is recommended.