B7-1/CD80, Rabbit anti-Human, Clone: IDEC-114 (Galiximab), Novus Biologicals™

Rabbit Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB016063	Novus Biologicals NBP2811000.025MG	0.025 mg
NB016064	Novus Biologicals NBP2811000.2MG	0.2 mg

Description

B7-1/CD80 Monoclonal specifically detects B7-1/CD80 in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Functional, Block/Neutralize.

Specifications

• Antigen: B7-1/CD80
• Concentration: 1 mg/mL
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, Functional Assay, Blocking Assay
• Conjugate: Unconjugated
• Host Species: Rabbit
• Research Discipline: Adaptive Immunity, B Cell Development and Differentiation Markers, Cytokine Research, Immunology, Signal Transduction
• Formulation: PBS
• Gene ID (Entrez): 941
• Immunogen: Galiximab was prepared by immunizing cynomolgus monkeys with recombinant B7-1/CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line (Hariharan K, 2013)
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Clone: IDEC-114 (Galiximab)
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, Functional, Block/Neutralize
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: Activation B7-1 antigen, B7, BB1, CD28LG1, CD28LGB7-1 antigen), CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
• Gene Symbols: CD80
• Isotype: IgG λ
• Purification Method: Protein A purified
• Test Specificity: Galiximab is a primatized mAb which consists of human constant and primate (cynomolgus macaque) variable regions and binds specifically to B7-1/CD80. B7-1/CD80 is a surface glycoprotein and a member of the B7 family of costimulatory molecules. B7-1/CD80 antigen regulates T cell activation (through interacting with CD28 or CD152) and is expressed transiently in antigen-presenting cells, T cells and normal B cells, and expressed consitutively on various subtypes of B-cell lymphomas.