Novus Biologicals™ B7-2/CD86 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Rat B7-2/CD86.

Specifications

• Assay Range: 4.7 pg/mL
• Assay Sensitivity: 4.69-300 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Rat B7-2/CD86.
• Target Species: Rat
• For Use With (Application): Sandwich ELISA
• Gene Alias: Activation B7-2 antigen, B70, B7-2, B7-2 antigen, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86 antigen, CD86 molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86
• Gene ID (Entrez): 942
• Gene Symbol: CD86
• Host Species: Mouse
• Kit Contents:
  Standard: Each vial contains 19 ng of recombinant rat B7-2/CD86.
  Capture Antibody: 0.4 mg/mL of mouse anti-rat B7-2/CD86 monoclonal antibody.
  Detection Antibody: 0.25 mg/mL mouse anti-B7-2/CD86 monoclonal antibody conjugated to horseradish-peroxidase (HRP).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, B Cell Development and Differentiation Markers, Cell Cycle and Replication, Dendritic Cell Markers, Diabetes Research, Immunology, Myeloid derived Suppressor Cell
• Storage Requirements: Storage is content dependent.
• Target: B7-2/CD86
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.3 ng/mL is recommended.