Bax Antibody (BAX/962), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2444200.1MG

Catalog No. NBP24442001

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Bax Monoclonal specifically detects Bax in Human, Monkey, Mouse (Negative), Rat (Negative) samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Bax
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Gene Accession No.: Q07812
• Host Species: Mouse
• Molecular Weight of Antigen: 21 kDa
• Quantity: 0.1 mg
• Research Discipline: Apoptosis, Cancer, Core ESC Like Genes, Stem Cell Markers, Tumor Suppressors
• Gene ID (Entrez): 581
• Target Species: Human, Monkey, Mouse (Negative), Rat (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: BAX/962
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4
• Immunogen: Recombinant full-length human BAX protein.
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
• Content And Storage: Store at 4C.
• Isotype: IgG1

For Research Use Only