Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. Bcl-2 Antibody (100/D5), Novus Biologicals™

Bcl-2 Antibody (100/D5), Novus Biologicals™
SDP

Catalog No. NBP21520001 Shop All R&D Systems Products
Change view
Click to view available options
Quantity:
0.1 mg
0.2 mg
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
NBP21520001 0.1 mg
NBP21520002 0.2 mg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. NBP21520001 Supplier Novus Biologicals Supplier No. NBP2152000.1MG
Add to Cart
Add to Cart

Description

Mouse Monoclonal Antibody has been used in 7 publications

Bcl-2 Monoclonal specifically detects Bcl-2 in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular).

Specifications

Antigen Bcl-2
Applications Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone 100/D5
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Formulation PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Accession No. P10415
Gene Alias apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
Gene Symbols BCL2
Host Species Mouse
Immunogen The synthetic peptide corresponding to amino acids range from 41-64 (GAAPAPGIFSSQPG-Cys), of human Bcl-2 was used as immunogen to generate the antibody; GenBank no. NP_000648.2 (Pezzella et al, 1990).
Molecular Weight of Antigen 25 kDa
Purification Method Protein G purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Tumor Suppressors
Primary or Secondary Primary
Gene ID (Entrez) 596
Test Specificity This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Reconstitution Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Target Species Human, Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
Show More Show Less

For Research Use Only

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.