Bcl-2 Antibody (BCL2/796) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2478520.2MG

Catalog No. NBP247852B

Description

Bcl-2 Monoclonal antibody specifically detects Antigen in Human samples. It is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting.

Specifications

• Antigen: Bcl-2
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
• Host Species: Mouse
• Molecular Weight of Antigen: 25.5 kDa
• Quantity: 0.2 mg
• Research Discipline: Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Oncogenes, Tumor Suppressors
• Gene ID (Entrez): 596
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: BCL2/796
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: BCL2
• Immunogen: Recombinant full-length human bcl-2 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ