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Bcl-2 Antibody (BCL2/796), Novus Biologicals™
SDP

Catalog No. NBP24478401 Shop All R&D Systems Products
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NBP24478401 0.1 mg
NBP24478400 0.02 mg
NBP24478402 0.2 mg
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Catalog No. NBP24478401 Supplier Novus Biologicals Supplier No. NBP2447840.1MG
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Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Bcl-2 Monoclonal specifically detects Bcl-2 in Human samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

Antigen Bcl-2
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone BCL2/796
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P10415
Gene Alias apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
Gene Symbols BCL2
Host Species Mouse
Immunogen Recombinant full-length human bcl-2 protein
Molecular Weight of Antigen 25.5 kDa
Purification Method Protein A or G purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Oncogenes, Tumor Suppressors
Primary or Secondary Primary
Gene ID (Entrez) 596
Test Specificity This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Target Species Human
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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