bcl-x Antibody (SPM337), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2444160.02MG

Catalog No. NBP24441600

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Bcl-xL Monoclonal specifically detects Bcl-xL in Human, Mouse, Rat, Porcine samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Bcl-xL
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: Q07817
• Gene Symbols: BCL2L1
• Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
• Content And Storage: Store at 4C.
• Isotype: IgG2a

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: SPM337
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: Apoptosis regulator Bcl-X, bcl2-L-1, BCL2-like 1, bcl-2-like protein 1, BCLXBCL2LBcl-X, bcl-xL, BCLXL, BCL-XL/S, bcl-xS, BCLXS, DKFZp781P2092
• Host Species: Mouse
• Molecular Weight of Antigen: 27 kDa
• Quantity: 0.02 mg
• Research Discipline: Apoptosis, Cancer, Tumor Suppressors
• Gene ID (Entrez): 598
• Target Species: Human, Mouse, Rat, Pig
• Form: Purified

For Research Use Only