Bcl-xL Mouse, Clone: 2H12, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus Biologicals NBP2329170.02MG

Catalog No. NBP232917F

Description

Bcl-xL Monoclonal antibody specifically detects Antigen in Human, Mouse, Porcine, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting.

Specifications

• Antigen: Bcl-xL
• Clone: 2H12
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Gene Accession No.: Q07817
• Gene Alias: Apoptosis regulator Bcl-X, bcl2-L-1, BCL2-like 1, bcl-2-like protein 1, BCLXBCL2LBcl-X, bcl-xL, BCLXL, BCL-XL/S, bcl-xS, BCLXS, DKFZp781P2092
• Host Species: Mouse
• Isotype: IgG2a
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Storage Requirements: Store at 4C.
• Monoclonal or Polyclonal: Monoclonal
• Test Specificity: Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Format: Purified
• Gene Symbols: BCL2L1
• Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein
• Molecular Weight of Antigen: 27 kDa
• Quantity: 0.02 mg
• Research Discipline: Apoptosis, Cancer, Phospho Specific, Tumor Suppressors
• Primary or Secondary: Primary
• Gene ID (Entrez): 598
• Target Species: Human, Mouse, Rat, Porcine