beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2477040.2MG

Catalog No. NBP247704B

Description

beta 2-Microglobulin Monoclonal antibody specifically detects Antigen in Human, Primate samples. It is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifications

• Antigen: beta 2-Microglobulin
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Flow (Cell Surface) 1:10-1:1000, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Gene Alias: beta chain of MHC class I molecules, beta-2-microglobin, beta-2-microglobulin
• Host Species: Mouse
• Molecular Weight of Antigen: 12 kDa
• Quantity: 0.2 mg
• Research Discipline: Adaptive Immunity, Cancer, Cell Biology, Diabetes Research, Immunology
• Gene ID (Entrez): 567
• Target Species: Human, Primate
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: B2M/961
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: B2M
• Immunogen: Full length recombinant human B2M protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2b κ