beta 2-Microglobulin Antibody (B2M/961) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247704A	Novus Biologicals NBP2477040.1MG	0.1 mg
NBP247704B	Novus Biologicals NBP2477040.2MG	0.2 mg

Description

beta 2-Microglobulin Monoclonal specifically detects beta 2-Microglobulin in Human, Primate samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Cell Surface), Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: beta 2-Microglobulin
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Cancer, Cell Biology, Diabetes Research, Immunology
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 567
• Immunogen: Full length recombinant human B2M protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 12 kDa

• Clone: B2M/961
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Flow (Cell Surface) 1:10-1:1000, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Primate
• Gene Alias: beta chain of MHC class I molecules, beta-2-microglobin, beta-2-microglobulin
• Gene Symbols: B2M
• Isotype: IgG2b κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.