beta-Actin Antibody (8H10D10) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 11 publications

Supplier:  Novus Biologicals NBP147423

Catalog No. NBP147423

Description

beta-Actin Monoclonal specifically detects beta-Actin in Human, Mouse, Rat, Chinese Hamster, Primate samples. It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoblotting.

Specifications

• Antigen: beta-Actin
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:1000-1:5000, Simple Western 12.5, Flow Cytometry 1:200-1:400, ELISA 1:10000, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunoblotting reported in scientific literature (PMID 27903531), Flow (Intracellular) 1:200 - 1:400
• Gene Accession No.: P60709
• Gene Symbols: ACTB
• Immunogen: This beta-Actin Antibody (8H10D10) was made to a synthetic peptide corresponding to amino-terminal residues of human Beta Actin, conjugated to KLH. [UniProt# P60709]
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Chinese Hamster, Primate
• Form: Precipitation

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoblot
• Clone: 8H10D10
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% Sodium Azide
• Gene Alias: ACTB, actin, beta, actin, cytoplasmic 1, B-Actin, Beta Actin, beta cytoskeletal actin, Beta-actin, BRWS1, I(2)-Actin, PS1TP5-binding protein 1, PS1TP5BP1
• Host Species: Mouse
• Molecular Weight of Antigen: 42 kDa
• Quantity: 0.1 mL
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Loading Controls, Signal Transduction, Stem Cell Markers
• Gene ID (Entrez): 60
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2b

For Research Use Only